@article{oai:uoeh-u.repo.nii.ac.jp:00000794,
 author = {濵砂, 良一 and 松本, 正広 and レ　ティ, ファン and 藤本, 直浩 and 松本, 哲朗 and Hamasuna, Ryoichi and Matsumoto, Masahiro and Le Thi,  Phuong and Fujimoto, Naohiro and Matsumoto, Tetsuro},
 issue = {1},
 journal = {産業医科大学雑誌, Journal of UOEH},
 month = {Mar},
 note = {Mycoplasma genitaliumは性感染症である男子尿道炎の原因微生物である．我が国では非淋菌性尿道炎患者の15-25%の尿から検出される．世界的にマクロライド耐性，キノロン耐性M. genitaliumの出現が，尿道炎治療を行う上で問題となっている．しかし，わが国ではM. genitaliumを検出するための保険適用を有する検査キットがない．今回，Seegene社の性感染症関連微生物7種同時検出キット（AnyplexTM II STI-7 Detection; Neisseria gonorrhoeae，Chlamydia trachomatis，M. genitalium，Mycoplasma hominis，Ureaplasma urealyticum，Ureaplasma parvum，Trichomonas vaginalisを検出）を用いて，M. genitaliumの検出に関する検討を行った．本キットの検出限界を検討するため，保存するM. genitalium 17株を用いた．SP4 mycoplasma mediumで増殖したM. genitalium培養液から，M. genitaliumのDNAを抽出し，M. genitaliumのDNAコピー数を測定した．M. genitaliumのDNA抽出液を101～108倍に希釈した希釈液を作成した．それぞれの希釈液をAnyplexTM II STI-7 Detectionで反応させ，もっとも希釈した液でM. genitaliumが検出されたものを検出限界とした．17株中14株で，希釈限界におけるDNAコピー数は10 genome equivalent（ geq）/reaction未満で，3株で10 geq/reaction以上であった．その中でもっとも高いDNAコピー数は48.4 geq/reactionであり，約50 geq/reactionがAnyplexTM II STI-7 Detectionにより保証されるM. genitaliumのDNAコピー数と考えられた．また，保存する尿検体からDNAを抽出し，MgPa geneの一部を増幅させるPCR法とAnyplexTM II STI-7 Detectionとで，M. genitaliumの検出率を比較した．両方法での陽性一致率は96.4%（27/28），陰性一致率は98.6%（ 71/72）であった．両方法との間に1例ずつの不一致があったものの，陽性，陰性一致率とも高かった．Seegene社の性感染症関連微生物7種同時検出キット，AnyplexTM II STI-7 Detectionを培養液，尿検体の面から検証し，M. genitalium検出に関して有用性が高いことが分かった．, Mycoplasma genitalium is one of the pathogenic microorganisms in male urethritis as a sexually transmitted infection (STI). M. genitalium is detected in the urine specimens of 15-25% male patients with urethritis. The emergence of macrolide- or fluoroquinolone-resistant M. genitalium has become a serious problem in the treatment of male urethritis worldwide, but there is no commercial-based detecting kits accepted by the national insurance in Japan. In this study, we tested the validity of a molecular kit for detecting seven microorganisms related to STI (AnyplexTM II STI-7 Detection which detects Neisseria gonorrhoeae, Chlamydia trachomatis, M. genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum, Trichomonas vaginalis) produced by Seegene company in Korea. Seventeen M. genitalium strains were used to determine the detection limit of M. genitalium. M. genitalium DNA samples were extracted from M. genitalium strains and the diluted DNA samples were reacted to detect M. genitalium by the AnyplexTM II STI-7 Detection. The detection limit was determined as the maximum dilution of DNA samples and the number of M. genitalium DNA copies calculated. In this study, the minimum DNA copies to detect M. genitalium by the AnyplexTM II STI-7 Detection was determined to be around 50 per reaction. The detection rates of M. genitalium in urine specimens were compared between MgPa gene PCR and the AnyplexTM II STI-7 Detection. The positive and negative concordant rates were high as 96.4% (27/28) and 98.6% (71/72), respectively. The validity of the kit for detecting seven microorganisms related to STI (AnyplexTM II STI-7 Detection) was high and thought to be useful for clinical uses.},
 pages = {45--52},
 title = {男子尿道炎からのMycoplasma genitalium検出のためのキットの検討},
 volume = {40},
 year = {2018}
}