{"created":"2023-06-20T15:46:09.450060+00:00","id":747,"links":{},"metadata":{"_buckets":{"deposit":"4aa0e369-618f-4404-809b-6a63b66eaa67"},"_deposit":{"created_by":4,"id":"747","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"747"},"status":"published"},"_oai":{"id":"oai:uoeh-u.repo.nii.ac.jp:00000747","sets":["13:24:31"]},"author_link":["2490","2489","2488","2494","2493","2495","2492","2491"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-09-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"232","bibliographicPageStart":"223","bibliographicVolumeNumber":"38","bibliographic_titles":[{"bibliographic_title":"産業医科大学雑誌"},{"bibliographic_title":"Journal of UOEH","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Culture-independent methods to detect microorganisms have been developed in parallel with traditional culture-based methods ever since the classification of bacteria based on 16S rRNA gene sequences was advocated in the 1970s. The development and the prevalence of culture-independent molecular technologies have provided revolutionary progress in microbial studies. The development of these technologies contributes significantly to the research of microorganisms that cannot be detected by traditional methods such as culture-dependent methods.Many molecular methods targeting the 16S rRNA gene, such as fluorescence in situ hybridization (FISH), quantitative PCR, terminal restriction fragment length polymorphism (T-RFLP), denaturing-gradient gel electrophoresis (DGGE), clone library analysis, and next-generation DNA sequencing (NGS) technologies, have been applied to various microbial studies. Notably, the advent of NGS technologies enabled a large-scale research of the bacterial community. Many recent studies using the NGS technologies have revealed that a larger number of bacteria and taxa than previously thought inhabit various parts of the human body and various places on the earth. The principles and characteristics of each molecular method are different, and each method possesses individual advantages; for example target specificity, comprehensiveness, rapidness, and cost efficiency. Therefore it is important that the methods used in studies are suitable for the objective and materials. Herein, we highlights molecular approaches targeting the 16S rRNA gene in bacterial community analysis, and focuses on the advantages and limitations of each technology.\n1970年代に16S rRNA遺伝子の塩基配列に基づく細菌の系統的な生物種分類法が提唱されて以来，微生物を検出するため，従来の培養法に加え，培養法に依存しない分子生物学的手法が考案されてきた．その開発と普及は微生物研究に革命的な進歩をもたらし，従来の培養法では検出できない細菌の研究に大きく寄与している．蛍光 in situ ハイブリダイゼーション法（FISH），定量PCR（Q-PCR），末端標識制限酵素断片多型分析（T-RFLP），変性剤濃度勾配ゲル電気泳動法（DGGE），クローンライブラリー解析や次世代型DNAシークエンス解析などの16S rRNA遺伝子を標的とした分子生物学的手法は，さまざまな微生物研究に応用されている．特に，次世代型DNAシークエンサーを用いた多くの研究は，大規模な細菌叢解析を可能にしており，最近の多くの研究は人体のさまざまな部位や，地球上のさまざまな場所に予想以上の数と種類の細菌が生息していることを明らかにしている．これらの分子生物学的手法は，それぞれの方法で原理や特徴は異なり，標的特異性，網羅性，迅速性や経済性などにおいて，それぞれ独自の利点を有している．それゆえ，研究の目的や対象に応じて，適した手法を選択することは重要である．本稿では，細菌叢解析に用いられる16S rRNA遺伝子を標的とした手法について概説し，それぞれの手法の利点や限界について考察する．","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"産業医科大学（産業医科大学学会）"}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Journal of UOEH　"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.uoeh-u.ac.jp/kouza/journal/intro_e.html","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"産業医科大学雑誌　"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.uoeh-u.ac.jp/kouza/journal/intro_j.html","subitem_relation_type_select":"URI"}}]},"item_10001_select_24":{"attribute_name":"記事区分","attribute_value_mlt":[{"subitem_select_item":"Review"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"ISSN　0387-821X(PRINT), ISSN 2187-2864(ONLINE)","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"福田  , 和正"},{"creatorName":"フクダ, カズマサ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2488","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小川 , みどり "},{"creatorName":"オガワ, ミドリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2489","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"谷口 , 初美 "},{"creatorName":"タニグチ, ハツミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2490","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"齋藤 , 光正"},{"creatorName":"サイトウ, ミツマサ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2491","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukuda, Kazumasa ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2492","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogawa, Midori ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2493","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Taniguchi, Hatsumi ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2494","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saito, Mitsumasa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2495","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-07-21"}],"displaytype":"detail","filename":"04-福田 和正.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"S00038304","url":"https://uoeh-u.repo.nii.ac.jp/record/747/files/04-福田 和正.pdf"},"version_id":"b5aadd4d-92af-4b55-87e1-27e591eb077f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"16S rRNA遺伝子","subitem_subject_scheme":"Other"},{"subitem_subject":"細菌叢解析","subitem_subject_scheme":"Other"},{"subitem_subject":"DNAシーケンス","subitem_subject_scheme":"Other"},{"subitem_subject":"16S rRNA gene","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bacterial community analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA sequencing","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"16S rRNA遺伝子を標的とした細菌叢解析手法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"16S rRNA遺伝子を標的とした細菌叢解析手法"},{"subitem_title":"Molecular Approaches to Studying Microbial Communities: Targeting the 16S Ribosomal RNA Gene","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"4","path":["31"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-08-03"},"publish_date":"2020-08-03","publish_status":"0","recid":"747","relation_version_is_last":true,"title":["16S rRNA遺伝子を標的とした細菌叢解析手法"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-06-20T15:58:44.581222+00:00"}